{"id":20508,"date":"2024-04-09T20:59:00","date_gmt":"2024-04-09T20:59:00","guid":{"rendered":"https:\/\/www.writemyessays.app\/blog\/questions\/laboratory-techniques-in-dna-manipulation-bioinformatics-pcr-cloning-and-dna-manipulation-laboratory-report\/"},"modified":"2024-04-09T20:59:00","modified_gmt":"2024-04-09T20:59:00","slug":"laboratory-techniques-in-dna-manipulation-bioinformatics-pcr-cloning-and-dna-manipulation-laboratory-report","status":"publish","type":"questions","link":"https:\/\/www.writemyessays.app\/blog\/questions\/laboratory-techniques-in-dna-manipulation-bioinformatics-pcr-cloning-and-dna-manipulation-laboratory-report\/","title":{"rendered":"Laboratory Techniques in DNA Manipulation Bioinformatics, PCR Cloning and DNA Manipulation Laboratory Report"},"content":{"rendered":"

Please complete the lab report as per the document instructions ! Pleaes no AI, and make sure to follow the instructions. it’s based on gene x<\/p>\n

<\/div>\n
I counted 1050 words total required, probably\/maybe some sources for support.<\/div>\n
there are 2 images from the results experiment day… (colony PCR)<\/div>\n
<\/div>\n
<\/div>\n
\n

MOD007185:Laboratory Techniques in DNA Manipulation<\/b><\/p>\n

Bioinformatics, PCR Cloning and DNA Manipulation Laboratory Report<\/b><\/p>\n


<\/p>\n


<\/p>\n

PART I: DNA cloning and Manipulation practical write up<\/b><\/p>\n


<\/p>\n

Complete the write-up of the cloning practical using the template and mark scheme specified below.  <\/span>Make sure that you include your SID, and save the file using your SID as the filename<\/b>.  <\/span>Do not include your name anywhere on the document.  <\/span>This section should be fully referenced. The write-up should include the following sections:<\/p>\n


<\/p>\n

Introduction: <\/b>300 words equivalent (20 marks)<\/b><\/p>\n

Provide a brief introduction to molecular cloning. Provide information on the uses of GST fusion proteins in in vitro<\/i> molecular biology experiments. <\/span><\/p>\n

<\/b>
<\/p>\n

Method: <\/b>75 words equivalent (5 Marks) <\/span><\/b><\/p>\n

Provide a methods section on Agarose gel electrophoresis (<\/b>Refer to the protocol on Canvas, only write anything in detail if the method was changed\/modified during the practical session)<\/p>\n

\t<\/span><\/p>\n

Results: <\/b>375 words equivalent (25 marks)<\/b> <\/span><\/p>\n

Provide a results section consisting of a brief written description of the results and annotated figures of the gels that you produced during the trimester. Include a figure legend for each figure.  <\/span><\/p>\n


<\/p>\n

Figure 1: Results of the amplification of GST DNA by PCR<\/p>\n

Figure 2: Results of Colony PCR <\/span><\/p>\n

Figure 3: Results restriction enzyme mapping of plasmid DNA<\/p>\n

Figure 4: Results showing analysis of expressed GST protein using SDS-PAGE <\/span><\/p>\n


<\/p>\n

Discussion: <\/b>300 words equivalent (20 marks)<\/b><\/p>\n

Discuss the experimental results provided in Figures 1-4.<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Please complete the lab report as per the document instructions ! Pleaes no AI, and make sure to follow the instructions. it’s based on gene x I counted 1050 words total required, probably\/maybe some sources for support. there are 2 images from the results experiment day… (colony PCR) MOD007185:Laboratory Techniques in DNA Manipulation Bioinformatics, PCR […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"closed","template":"","meta":[],"disciplines":[40],"paper_types":[],"tagged":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.writemyessays.app\/blog\/wp-json\/wp\/v2\/questions\/20508"}],"collection":[{"href":"https:\/\/www.writemyessays.app\/blog\/wp-json\/wp\/v2\/questions"}],"about":[{"href":"https:\/\/www.writemyessays.app\/blog\/wp-json\/wp\/v2\/types\/questions"}],"author":[{"embeddable":true,"href":"https:\/\/www.writemyessays.app\/blog\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.writemyessays.app\/blog\/wp-json\/wp\/v2\/comments?post=20508"}],"version-history":[{"count":0,"href":"https:\/\/www.writemyessays.app\/blog\/wp-json\/wp\/v2\/questions\/20508\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.writemyessays.app\/blog\/wp-json\/wp\/v2\/media?parent=20508"}],"wp:term":[{"taxonomy":"disciplines","embeddable":true,"href":"https:\/\/www.writemyessays.app\/blog\/wp-json\/wp\/v2\/disciplines?post=20508"},{"taxonomy":"paper_types","embeddable":true,"href":"https:\/\/www.writemyessays.app\/blog\/wp-json\/wp\/v2\/paper_types?post=20508"},{"taxonomy":"tagged","embeddable":true,"href":"https:\/\/www.writemyessays.app\/blog\/wp-json\/wp\/v2\/tagged?post=20508"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}